Antitumor effect of proanthocyanidin induced apoptosis in human colorectal cancer (HT-29) cells and its molecular docking studies.


PMID: 

BMC Chem. 2019 Dec ;13(1):21. Epub 2019 Feb 4. PMID: 31384770

Abstract Title: 

Antitumor effect of proanthocyanidin induced apoptosis in human colorectal cancer (HT-29) cells and its molecular docking studies.

Abstract: 

Proanthocyanidin (PAC) is a promising compound that has displayed its potent antineoplastic properties with a specific intrinsic pathway. This precise us to explore the phyto-preventive effect of PAC against colon cancer (HT-29). The results showed that PAC inhibited the cell growth and GIvalue was found to be 6.25 μM for 24 h exposure, when correlated to the normal cell line does not have toxicity was noticed. The linguistic differences, similarly membrane blebbing, cell shrinkage fragmented nuclear bodies and mitochondrial membrane were observed in AO/EtBr and DAPI staining. The features of regular mechanical apoptotic characterization was analyzed by DNA fragmentation. The cell cycle arrest at G2/M phases was detected using FACS analysis. The early and late apoptotic cells were observed by using Annexin V/PI staining. The ligand-protein interaction and docking studies were performed using Schrodinger's software. The QPLD analysis of docking studies revealed that PAC exhibited better binding affinity of – 5.23, – 5.17 and – 4.43, – 4.47 kcal/mol against BCL-XL, CDK2 and were compared with 5-FU respectively, …

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Anticancer effects of five biflavonoids from Ginkgo biloba flowers In vitro.


PMID: 

Molecules. 2019 Apr 16 ;24(8). Epub 2019 Apr 16. PMID: 30995808

Abstract Title: 

Anticancer Effects of Five Biflavonoids fromL. Male Flowers In Vitro.

Abstract: 

L., an ancient dioecious gymnosperm, is now cultivated worldwide for landscaping and medical purposes. A novel biflavonoid-amentoflavone 7''-O-β-D-glucopyranoside ()-and four known biflavonoids were isolated and identified from the male flowers of. The anti-proliferative activities of five biflavonoids were evaluated on different cancer lines. Bilobetin () and isoginkgetin () exhibited better anti-proliferative activities on different cancer lines. Their effects were found to be cell-specific and in a dose and time dependent manner for the most sensitive HeLa cells. The significant morphological changes validated their anticancer effects in a dose-dependent manner. They were capable of arresting the G2/M phase of the cell cycle, inducing the apoptosis of HeLa cells dose-dependently and activating the proapoptotic protein Bax and the executor caspase-3. Bilobetin () could also inhibit the antiapoptotic protein Bcl-2. These might be the mechanism underlying their anti-proliferation. In short, bilobetin () and isoginkgetin () might be the early lead compounds for new anticancer agents.

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